The activity of Im-LMS was almost completely stable after being stored for 10 days. Following eight reuse cycles of Im-LMS for dye treatment, a decolorization yield of 79% was obtained. ![]() Malachite green solution was detoxified completely after biodegradation by Im-LMS. The decolorization yields of malachite green by Im-LMS and the free laccase-mediator system were 92% within 120 min and 90% within 90 min. ![]() The catalytic activity of Im-LMS was evaluated for dye decolorization using malachite green. Here, a novel biocatalyst (Im-LMS) was fabricated by immobilizing both laccase and a mediator (2,2'-azino-bis-6-sulfonic acid) on layered double hydroxide/alginate biohybrid beads. The application of laccase-mediator-based catalysis is limited owing to the high cost of laccases and mediators and the potential toxicity of free mediators.
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